enos protein  (Servicebio Inc)

Journal: Brain and Behavior

Article Title: Propofol Suppresses Ferroptosis via Modulating eNOS/NO Signaling Pathway to Improve Traumatic Brain Injury

doi: 10.1002/brb3.70187

Figure Lengend Snippet: The neuroprotective effect of propofol may be associated with promoting eNOS to produce nitric oxide (NO). (A) Representative immunoblots of eNOS and α‐tubulin proteins from four groups of mice. (B) Comparative analysis of eNOS protein expression among the four groups of mice ( n = 6). (C) Comparison of NO concentrations in brain tissue among the four groups of mice ( n = 6). Data are presented as mean ± standard deviation and were statistically analyzed using ANOVA, with p values indicated on the bar charts.

Article Snippet: In subsequent experiments, before the application of propofol, eNOS protein inhibitor L‐NAME (S2877, Selleck, USA) was administered intraperitoneally at a dose of 30 mg/kg, or an equivalent volume of solvent was injected as a control.

Techniques: Western Blot, Expressing, Comparison, Standard Deviation

Journal: Brain and Behavior

Article Title: Propofol Suppresses Ferroptosis via Modulating eNOS/NO Signaling Pathway to Improve Traumatic Brain Injury

doi: 10.1002/brb3.70187

Figure Lengend Snippet: The eNOS protein inhibitor L‐NAME can reverse the early neuroprotective effects of propofol on TBI. (A) Representative images of Perls staining from two groups of mice are displayed. (B) A comparison of the number of positively stained cells in Perls staining between the two groups of mice ( n = 6 per group) is presented. (C) Representative images of Nissl staining from two groups of mice are shown, with scale bars at 50 or 10 µm. (D, E) Bar graphs illustrate the statistical analysis of Nissl body count and positive area per field of view for four groups of mice ( n = 6 per group). (F) Representative TUNEL fluorescence images from the damaged cortical area, hippocampal CA1 region, CA3 region, and DG region of two groups of mice are displayed. Red labeling indicates neurons, green labeling indicates apoptotic cells, and blue labeling indicates cell nuclei, with a scale bar at 50 µm. (G) A comparison of the number of apoptotic neurons in the damaged cortical area, hippocampal CA1 region, CA3 region, and DG region among four groups of mice ( n = 6 per group) is presented. (H) Representative immunoblots of Gpx4, 4‐HNE, Bak, Bcl‐2, and α‐tubulin proteins from two groups of mice are shown. (I–K) Comparisons of Gpx4 protein expression, 4‐HNE protein expression, and the Bak/Bcl‐2 ratio between the two groups of mice ( n = 6 per group) are provided. (L) Comparison of the expression levels of FTH1 mRNA between the two groups of mice ( n = 6 per group). Data are presented as mean ± standard deviation and statistically analyzed using Student's t ‐test, with p values on the bar charts.

Article Snippet: In subsequent experiments, before the application of propofol, eNOS protein inhibitor L‐NAME (S2877, Selleck, USA) was administered intraperitoneally at a dose of 30 mg/kg, or an equivalent volume of solvent was injected as a control.

Techniques: Staining, Comparison, TUNEL Assay, Fluorescence, Labeling, Western Blot, Expressing, Standard Deviation

Journal: Brain and Behavior

Article Title: Propofol Suppresses Ferroptosis via Modulating eNOS/NO Signaling Pathway to Improve Traumatic Brain Injury

doi: 10.1002/brb3.70187

Figure Lengend Snippet: The eNOS protein inhibitor L‐NAME can reverse the effect of propofol in improving long‐term outcomes after TBI. (A, B) Representative GFAP and Iba1 immunofluorescence images from the damaged cortical area, hippocampal CA1 region, CA3 region, and DG region of two groups of mice are shown. (C, D) Comparisons of GFAP‐positive area and Iba1‐positive cell counts in the damaged cortical area, hippocampal CA1 region, CA3 region, and DG region between two groups of mice are presented ( n = 6 per group). (E) The escape latencies during the MWM training period for two groups of mice were compared, and two‐way repeated measures ANOVA was used for statistical analysis, with **** p < 0.0001. (F) Representative traces of the mice in the MWM are shown. (G, H) Comparisons of platform crossing times and time spent in the target quadrant between two groups of mice are presented ( n = 6 per group). (I) Representative traces of the mice in the NOR test are shown, with N representing the novel object and F representing the familiar object. (J) A comparison of the discrimination index between two groups of mice is presented ( n = 6 per group). Data are expressed as mean ± standard deviation and were statistically analyzed using Student's t ‐test, with p values indicated on the bar charts.

Article Snippet: In subsequent experiments, before the application of propofol, eNOS protein inhibitor L‐NAME (S2877, Selleck, USA) was administered intraperitoneally at a dose of 30 mg/kg, or an equivalent volume of solvent was injected as a control.

Techniques: Immunofluorescence, Comparison, Standard Deviation

Journal: Brain and Behavior

Article Title: Propofol Suppresses Ferroptosis via Modulating eNOS/NO Signaling Pathway to Improve Traumatic Brain Injury

doi: 10.1002/brb3.70187

Figure Lengend Snippet: The neuroprotective effect of propofol may be associated with promoting eNOS to produce nitric oxide (NO). (A) Representative immunoblots of eNOS and α‐tubulin proteins from four groups of mice. (B) Comparative analysis of eNOS protein expression among the four groups of mice ( n = 6). (C) Comparison of NO concentrations in brain tissue among the four groups of mice ( n = 6). Data are presented as mean ± standard deviation and were statistically analyzed using ANOVA, with p values indicated on the bar charts.

Article Snippet: In subsequent experiments, before the application of propofol, eNOS protein inhibitor L‐NAME (S2877, Selleck, USA) was administered intraperitoneally at a dose of 30 mg/kg, or an equivalent volume of solvent was injected as a control.

Techniques: Western Blot, Expressing, Comparison, Standard Deviation

Journal: Brain and Behavior

Article Title: Propofol Suppresses Ferroptosis via Modulating eNOS/NO Signaling Pathway to Improve Traumatic Brain Injury

doi: 10.1002/brb3.70187

Figure Lengend Snippet: The eNOS protein inhibitor L‐NAME can reverse the early neuroprotective effects of propofol on TBI. (A) Representative images of Perls staining from two groups of mice are displayed. (B) A comparison of the number of positively stained cells in Perls staining between the two groups of mice ( n = 6 per group) is presented. (C) Representative images of Nissl staining from two groups of mice are shown, with scale bars at 50 or 10 µm. (D, E) Bar graphs illustrate the statistical analysis of Nissl body count and positive area per field of view for four groups of mice ( n = 6 per group). (F) Representative TUNEL fluorescence images from the damaged cortical area, hippocampal CA1 region, CA3 region, and DG region of two groups of mice are displayed. Red labeling indicates neurons, green labeling indicates apoptotic cells, and blue labeling indicates cell nuclei, with a scale bar at 50 µm. (G) A comparison of the number of apoptotic neurons in the damaged cortical area, hippocampal CA1 region, CA3 region, and DG region among four groups of mice ( n = 6 per group) is presented. (H) Representative immunoblots of Gpx4, 4‐HNE, Bak, Bcl‐2, and α‐tubulin proteins from two groups of mice are shown. (I–K) Comparisons of Gpx4 protein expression, 4‐HNE protein expression, and the Bak/Bcl‐2 ratio between the two groups of mice ( n = 6 per group) are provided. (L) Comparison of the expression levels of FTH1 mRNA between the two groups of mice ( n = 6 per group). Data are presented as mean ± standard deviation and statistically analyzed using Student's t ‐test, with p values on the bar charts.

Article Snippet: In subsequent experiments, before the application of propofol, eNOS protein inhibitor L‐NAME (S2877, Selleck, USA) was administered intraperitoneally at a dose of 30 mg/kg, or an equivalent volume of solvent was injected as a control.

Techniques: Staining, Comparison, TUNEL Assay, Fluorescence, Labeling, Western Blot, Expressing, Standard Deviation

Journal: Brain and Behavior

Article Title: Propofol Suppresses Ferroptosis via Modulating eNOS/NO Signaling Pathway to Improve Traumatic Brain Injury

doi: 10.1002/brb3.70187

Figure Lengend Snippet: The eNOS protein inhibitor L‐NAME can reverse the effect of propofol in improving long‐term outcomes after TBI. (A, B) Representative GFAP and Iba1 immunofluorescence images from the damaged cortical area, hippocampal CA1 region, CA3 region, and DG region of two groups of mice are shown. (C, D) Comparisons of GFAP‐positive area and Iba1‐positive cell counts in the damaged cortical area, hippocampal CA1 region, CA3 region, and DG region between two groups of mice are presented ( n = 6 per group). (E) The escape latencies during the MWM training period for two groups of mice were compared, and two‐way repeated measures ANOVA was used for statistical analysis, with **** p < 0.0001. (F) Representative traces of the mice in the MWM are shown. (G, H) Comparisons of platform crossing times and time spent in the target quadrant between two groups of mice are presented ( n = 6 per group). (I) Representative traces of the mice in the NOR test are shown, with N representing the novel object and F representing the familiar object. (J) A comparison of the discrimination index between two groups of mice is presented ( n = 6 per group). Data are expressed as mean ± standard deviation and were statistically analyzed using Student's t ‐test, with p values indicated on the bar charts.

Article Snippet: In subsequent experiments, before the application of propofol, eNOS protein inhibitor L‐NAME (S2877, Selleck, USA) was administered intraperitoneally at a dose of 30 mg/kg, or an equivalent volume of solvent was injected as a control.

Techniques: Immunofluorescence, Comparison, Standard Deviation